Optimization and Validation of an Extraction Method for Endosulfan Lactone on a Solid Substrate

Endosulfan lactone is a metabolite obtained from the biological oxidation of insecticide endosulfan by action microorganisms present in soil. This more toxic and persistent than parent compound. Therefore, it extremely important to be able determine presence this However, accessible methods for extraction soil were not found published literature. For reason, aim study was evaluate two conventional liquid–solid determination solid substrate using solvents (ethyl acetate acetonitrile) HPLC UV-VIS. The acetonitrile rotary agitation method one with highest efficiency (97%), optimized factorial 32 response surface design, validated terms linearity precision. shown r > 0.999 wide spike level (0.15–100 mg kg?1), detection limit (DL) 0.045 kg?1 quantification (QL) 0.15 kg?1. efficient that used ethyl acetate, so could extract quantify agricultural